出處

Int. J. Cancer: 70,699-705 (1997)
-1997 Wiley-Liss, Inc.

題目

THE ANTI-TUMOR EFFECT OF GANODERMA LUCIDUM IS MEDIATED BY CYTOKINES RELEASED FROM ACTIVATED MACROPHAGES AND T LYMPHOCYTES

作者

Sheng-Yuan Wang, Ming-Ling Hsu, Hui-Chi Hsu, Cheng-Hwai Tzeng, Shiuh-Sheng Lee, Ming-Shi Shiao and Chi-Kuan Ho
Department of Medical Research, Veterans General Hospital-Taipei, Taipei, Taiwan Republic of China
Department of Medicine, Veterans General Hospital-Taipei, Taipei, Taiwan Republic of China
Graduate Institute of Microbiology and Immunology, National Yang-Ming University, Taipei, Taiwan Republic of China
Department of Biochemistry, National Yang-Ming University, Taipei, Taiwan Republic of China
Center for Immunology, National Yang-Ming University, Taipei, Taiwan Republic of China

The present study was to ascertain the immunomodulating and anti-tumor effects of Ganoderma(G.) lucidum. Polysaccharides (PS) from fresh fruiting bodies of G. lucidum (PS-G) were isolated and used to potentiate cytokine production by human monocytes-macrophages and T lymphocytes. Our results had shown that the levels of interleukin (IL)-Iβ, tumor necrosis factor (INF)-α,and IL-6 in macrophage cultures treated with PS-G (100 μg/ml) were 5.1-, 9.8- and 29-fold higher, respectively, than those of untreated controls. In addition, the release of interferon(IFN)-γ from T lymphocytes was also greatly promoted in the presence of PS-G (25-100μg/ml). Furthermore, these cytokine-containing mononuclear cell-conditioned media (PSG-MNC-CM) were found to suppress the proliferation and clonogenicity of both the HL-60 and the U937 leukemic cell lines. DNA labeling and gel electrophoresis showed that treatment with PSG-MNC-CM markedly induced leukemic-cell apoptosis. Flow-cytometric analysis revealed that few (2.3±0.8%) apoptotic cells were seen in the control cultures, while PSG-MNC-CM treatment resulted in a significant increase in the apoptoic population both in the HL-60(38.3±4.5%) and in the U937(44.5±3.8%) cells. In addition, 40 to 45% of the treated leukemic cells were triggered to differentiate into mature monocytic cells expressing CD14 and CD68 surface antigens. However, PS-G alone had no such effects even at a higher does of 400μg/ml. Since untreated macrophages and T lymphocytes produced little or no cytokine, and normal MNC-CM did not suppress leukemic cell growth, it was suggestive that the anti-tumor activity of PSG-MNC-CM was derived from the elevated levels of cytokines. Antibody-neutralization studies further revealed that the anti-tumor cytokines in the PSG-MNC-CM were mainly of INF-α and IFN-γ, and these 2 cytokines acted synergistically on the inhibition of leukemic-cell growth. Int. J. Cancer: 70,699-705, 1997
？ 1997 Wiley-Liss, Inc.